No conjugate is needed for fluorescent blot detection. Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3, 0.01% bromophenol blue.įluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. Packaging Options Number of Applications (Volume) Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range.Molecular weight sizing of proteins after blot development.Monitoring blot transfer efficiency with easy orientation via multicolor standards.The protein standards are supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. Thermo Scientific Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120 kDa) for use as size standards in protein electrophoresis (SDS-PAGE). Pink bands can be visualized with a 532 nm laser or green LED.Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. Fluorescent detection - blue bands can be visualized with a 635 nm laser or red LED.Strep-tag affinity peptide for detection and MW determination on western blots.Prestained bands for monitoring of electrophoresis progression and assessment of blotting efficiency.Dual-color prestained bands (reference bands prestained pink, all others prestained blue) The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 240 kDa. Amersham ECL DualVue Western Blotting Markers (15 000150 000 Da) RPN810 Amersham ECL Plex Fluorescent Rainbow Markers (12 000225 000 Da) RPN850E, RPN851E Amersham ECL Rainbow Marker - Low Range, (350040 000 Da) RPN755E (8 protein ladder) Amersham ECL Rainbow Marker - High Range, (12 000225 000 Da) RPN756E (10 protein ladder) 1.Proprietary staining technology that provides batch-to-batch MW consistency and reproducible electrophoretic migration.Highly accurate MW estimation, r 2 values >0.99 iBright Prestained Protein Ladder Click image to enlarge Request Sample: MagicMark XP Western Protein Standard Click image to enlarge: Choose for: Exceptional versatility for visible, IgG or fluorescent western blot detection: IgG binding for direct visualization during detection of western blot: Range (kDa) 11250: 20220: Number of bands.Clean, sharp bands with MW confirmed by mass spectrometry.Features and Benefits of WesternC Western Blotting Protein Standards These protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD and include three high-intensity reference bands (25, 50, and 75 kD). WesternC Standards for protein electrophoresis and blotting offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. Precision Plus Protein™ WesternC™ Protein Standards are prestained, broad-range protein standards that enable chemiluminescent detection when probed with StrepTactin-HRP conjugates, so the protein standard appears directly on a film or CCD image. Read their story and request a free set of matching (temporary) tattoos here. Two students celebrated their love of science with Precision Plus Protein Kaleidoscope Ladder tattoos.
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